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Beilstein J. Org. Chem. 2013, 9, 544–556, doi:10.3762/bjoc.9.60
Graphical Abstract
Figure 1: Design of the biotinylated Hsp90 probes based on PU-H71 (1a).
Scheme 1: Reagents and conditions: (a) D-biotin, DCC, DMAP, CH2Cl2, sonicate; (b) EZ-Link® NHS-LC-Biotin, DIE...
Scheme 2: Reagents and conditions: (a) 6-Boc-aminocaproic acid, DCC, DMAP, CH2Cl2, rt; (b) TFA, CH2Cl2, rt; (...
Figure 2: Analysis of the affinity and selectivity of the biotinylated probes for Hsp90. (a) K562 cancer cell...
Figure 3: Use of probe 2g to detect oncogenic Hsp90 by flow cytometry (a) and (b) and by microscopy (c). For ...